imaris 3d simulation  (Oxford Instruments)

Journal: Nature Communications

Article Title: Dextran-based T-cell expansion nanoparticles for manufacturing CAR T cells with augmented efficacy

doi: 10.1038/s41467-025-67868-1

Figure Lengend Snippet: a mCherry labelled Cathepsin L is located in Jurkat cell granules as shown by confocal imaging, scale bar: 5 μm, representative confocal images from 3 independent experiments performed with similar imaging results. b Engineered Jurkats were incubated with T-Expand or naked NPs both loaded with BV421-streptavidin for 24 h and then imaged by Airyscan confocal imaging (2D-view), scale bar: 5 µm. White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 µm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between Dynabeads™ and T-Expand. White circle indicates the location of T-Expand or Dynabeads TM , scale bar 3 µm. b Was created with BioRender.com released under a Creative Commons Attribution (CC BY) 4.0 International license. Significance was defined as p < 0.05, ns, not significant.

Article Snippet: White circle indicates the location of naked NPs or T-Expand. c Specific activation test, Airyscan imaging (3D view) and Imaris 3D simulation on how T-Expand interacting with engineered Jurkats, scale bar 2 μm. d , The distance of naked NPs or T-Expand to mCherry-Cathepsin L under Imaris 3D simulation, (number of granules n = 130), two tailed unpaired t test, and significance was defined as p < 0.05. e The real - time 3D confocal imaging indicates the granules cluster formation between DynabeadsTM and T-Expand.

Techniques: Imaging, Incubation, Activation Assay, Two Tailed Test

Journal: Research

Article Title: Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow

doi: 10.34133/research.0338

Figure Lengend Snippet: PTMS-FLOW technology enhances iPSC generation by cell fate selection. (A) UMAP embedding showing cell types of the selected colonies through manual selection. NPCs: neuron progenitor cells, iPSCs: induced pluripotent stem cells. (B) Immunofluorescence staining of reprogrammed colonies on Day 15. Top: FZD3 (green), OCT4 (red), and DAPI (blue); bottom: NOVA1 (green), OCT4 (red), and DAPI (blue). The confocal 3-dimensional (3D) datasets were processed with IMARIS software for 3D view; scale bar is 100 μm. (C) Schematic representation of the dual selection mode to obtain colonies. (D) Bright-field images of selected colonies using the dual selection mode. Scale bar is 200 μm. (E) OCT4 staining of selected colonies (left: single selection mode; right: dual selection mode; the OCT4 positive is represented iPSCs); High and Medium represented the fluorescence value of OCT4 staining at the high level and middle level in the corresponding selection mode, respectively. The data on the right side is the quantitative analysis of the fluorescent value of the OCT4 staining. **** P < 0.0001; scale bar is 200 μm. (F) UMAP plot analysis of the cell types from single run selection and dual run selection. (G) Comparison of the proportion of cell types (NPCs, iPSCs, Pre-neuron 1, Pre-neuron 2, Cell-cycle cells, and others) among manual selection mode, single run selection mode, and dual run selection mode.

Article Snippet: Multiple images were captured using LSM 800 (Zeiss) and 3D simulation was performed with Imaris (Oxford Instruments).

Techniques: Selection, Immunofluorescence, Staining, Software, Fluorescence, Comparison